A digital microfluidic interface between solid-phase microextraction and liquid chromatography-mass spectrometry

Choi K., Boyaci E., Kim J., Seale B., Barrera-Arbelaez L., Pawliszyn J., ...Daha Fazla

JOURNAL OF CHROMATOGRAPHY A, cilt.1444, ss.1-7, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1444
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.chroma.2016.03.029
  • Dergi Adı: JOURNAL OF CHROMATOGRAPHY A
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1-7
  • Anahtar Kelimeler: Digital microfluidics, Solid-phase microextraction, HPLC-MS, SPME, Urine, Steroid, Hormone, CAPILLARY ELECTROPHORETIC ZONES, QUANTITATIVE-ANALYSIS, DIELECTRIC ACTUATION, ESTROGEN METABOLITES, SAMPLE PREPARATION, FREE TESTOSTERONE, PERFORMANCE, EXTRACTION, URINE, DROPLETS
  • Orta Doğu Teknik Üniversitesi Adresli: Hayır

Özet

We introduce a method to couple solid-phase microextraction (SPME) with HPLC-MS using digital microfluidics (DMF). In the new system, SPME fibers are used to extract analytes from complex sample solutions, after which the analytes are desorbed into solvent droplets in a DMF device. The open geometry of DMF allows straightforward insertion of SPME fibers without requiring a complicated interface, and automated droplet manipulation enables multiplexed processing of the fibers. In contrast to other multiplexed SPME elution interfaces, the low volumes inherent to DMF allow for pre-concentration of analytes prior to analysis. The new SPME-DMF-HPLC-MS method was applied to the quantification of pg/mL-level free steroid hormones in urine. We propose that this new method will be useful for a wide range of applications requiring cleanup and pre-concentration with convenient coupling to high-performance analytical techniques. (C) 2016 Elsevier B.V. All rights reserved.