Sterically stabilized cationic liposomes improve the uptake and immunostimulatory activity of CpG oligonucleotides

Creative Commons License

Gursel I., Gursel M., Ishii K., Klinman D.

JOURNAL OF IMMUNOLOGY, vol.167, no.6, pp.3324-3328, 2001 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 167 Issue: 6
  • Publication Date: 2001
  • Doi Number: 10.4049/jimmunol.167.6.3324
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.3324-3328
  • Middle East Technical University Affiliated: Yes


Immunostimulatory CpG oligonucleotides (ODN) show promise as immune adjuvants, anti-allergens, and immunoprotective agents. Increasing the bioavailability and duration of action of CpG ODN should improve their therapeutic utility. Encapsulating ODN in sterically stabilized cationic liposomes provides protection from serum nucleases while facilitating uptake by B cells, dendritic cells, and macrophages. In a pathogen challenge model, sterically stabilized cationic liposomes encapsulation doubled the duration of CpG ODN-induced immune protection. In an immunization model, coencapsulation of CpG ODN with protein Ag (OVA) magnified the resultant Ag-specific IFN-gamma and IgG responses by 15- to 40-fold compared with Ag plus CpG ODN alone. These findings support the use of sterically stabilized cationic liposomes to significantly enhance the therapeutic efficacy of CpG ODN.