Phenol biosensor based on electrochemically controlled integration of tyrosinase in a redox polymer

Yildiz H. B., Castillo J., Guschin D. A., Toppare L., Schuhmann W.

MICROCHIMICA ACTA, cilt.159, ss.27-34, 2007 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 159
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1007/s00604-007-0768-1
  • Dergi Adı: MICROCHIMICA ACTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.27-34
  • Anahtar Kelimeler: tyrosinase, phenol, catechol, electrodeposition paint, redox polymer, substrate recycling, amperometric biosensor, ascorbic acid, interference elimination, IMMOBILIZATION MATRIX, ELECTRODES, ENZYME, DEPOSITION, CATECHOL, LIBRARY, FILMS
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

An amperometric biosensor for the detection of phenolic compounds was developed based on the immobilization of tyrosinase within an Os-complex functionalized electrodeposition polymer. Integration of tyrosinase within the redox polymer assures efficient catechol recycling between the enzyme and the polymer bound redox sites. The non-manual immobilization procedure improves the reproducibility of fabrication process, greatly reduces the desorption of the enzyme from the immobilization layer, and, most importantly prevents fast inactivation of the enzyme by its substrate due to fast redox cycling.