Phenol biosensor based on electrochemically controlled integration of tyrosinase in a redox polymer


Yildiz H. B. , Castillo J., Guschin D. A. , Toppare L. , Schuhmann W.

MICROCHIMICA ACTA, vol.159, pp.27-34, 2007 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 159
  • Publication Date: 2007
  • Doi Number: 10.1007/s00604-007-0768-1
  • Title of Journal : MICROCHIMICA ACTA
  • Page Numbers: pp.27-34

Abstract

An amperometric biosensor for the detection of phenolic compounds was developed based on the immobilization of tyrosinase within an Os-complex functionalized electrodeposition polymer. Integration of tyrosinase within the redox polymer assures efficient catechol recycling between the enzyme and the polymer bound redox sites. The non-manual immobilization procedure improves the reproducibility of fabrication process, greatly reduces the desorption of the enzyme from the immobilization layer, and, most importantly prevents fast inactivation of the enzyme by its substrate due to fast redox cycling.