A method for Red Blood Corpuscles (RBCs) counting has been developed using RBCs light microscopic images and Matlab algorithm. The Dataset consists of Red Blood Corpuscles (RBCs) images and there RBCs segmented images. A detailed description using flow chart is given in order to show how to produce RBCs mask. The RBCs mask was used to count the number of RBCs in the blood smear image. (C) 2015 The Authors. Published by Elsevier Inc.