Photoproduction of hydrogen from sugar refinery wastewater by Rhodobacter sphaeroides OU 001

Yetis M., Gunduz U., Eroglu I., Yucel M., Turker L.

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, vol.25, no.11, pp.1035-1041, 2000 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 11
  • Publication Date: 2000
  • Doi Number: 10.1016/s0360-3199(00)00027-6
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1035-1041
  • Keywords: biological hydrogen production, photobioreactor, Rhodobacter sphaeroides, PHOTOSYNTHETIC BACTERIA, WATER, CULTURE, RATES
  • Middle East Technical University Affiliated: Yes


Pretreated sugar refinery wastewater (SRWW) was used for the production of hydrogen by Rhodobacter sphaeroides O.U.001 in a 0.4 1 column photobioreactor. Hydrogen was produced at a rate of 0.001 1 hydrogen/h/l culture in 20% dilution of SRWW. To adjust the carbon concentration to 70 mM and nitrogen concentration to 2 mM, sucrose or l-malic acid was added as carbon source and sodium glutamate was added as nitrogen source to the 20% dilution of SRWW. By these adjustments, hydrogen production rate was increased to 0.005 1 hydrogen/h/l culture. Continuous hydrogen production was achieved in the same medium for 100 days at three different dilution rates. The maximum hydrogen produced was 2.67 1 tin 100 days) at a dilution rate of 0.0013 h(-1) (C) 2000 international Association for Hydrogen Energy. Published by Elsevier Science Ltd. All rights reserved.