Biological hydrogen production from olive mill wastewater with two-stage processes

Eroglu E., Eroglu I., Gunduz U., Turker L., Yucel M.

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, vol.31, no.11, pp.1527-1535, 2006 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 11
  • Publication Date: 2006
  • Doi Number: 10.1016/j.ijhydene.2006.06.020
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1527-1535
  • Keywords: biological hydrogen production, olive mill wastewater, clay treatment, coupled system, photofermentation, dark fermentation, RHODOBACTER-SPHAEROIDES, PHOTOSYNTHETIC BACTERIUM, PHOTOPRODUCTION, FERMENTATION
  • Middle East Technical University Affiliated: Yes


In the present work two novel two-stage hydrogen production processes from olive mill wastewater (OMW) have been introduced. The first two-stage process involved dark-fermentation followed by a photofermentation process. Dark-fermentation by activated sludge cultures and photofermentation by Rhodobacter sphaeroides O.U.001 were both performed in 55 ml glass vessels, under anaerobic conditions. In some cases of dark-fermentation, activated sludge was initially acclimatized to the OMW to provide the adaptation of microorganisms to the extreme conditions of OMW. The highest hydrogen production potential obtained was 291H(2)/l(OMW) after photofermentation with 50% (v/v) effluent of dark fermentation with activated sludge. Photofermentation with 50% (v/v) effluent of dark fermentation with acclimated activated sludge had the highest hydrogen production rate (0.00811(-1) h(-1)).