The aim of this study was to examine the masculinity and femininity scales of Bern Sex Role Inventory (BSRI) among Turkish university students. Five hundred thirty-six students (280 men and 256 women) volunteered to complete the short-form of the BSRI and answer demographic questions. In factor analyses, the original factor structure (Bem, 1981) was found both in the men's and women's data. Comparisons of the factor structures with target rotation (Procrustes rotation) and comparison indexes showed no difference between the factor structures found among men and women. The internal consistency of the masculinity and femininity scales was acceptable, and t-tests showed that women scored higher on the femininity scale, and men scored higher on the masculinity scale. There were significant differences between men and women only on two masculinity items, but significant differences were found in 8 (of 10) femininity items.