Immobilization of glucose oxidase in conducting graft copolymers and determination of glucose amount in orange juices with enzyme electrodes

Yildiz H., Kiralp S., Toppare L., Yagci Y.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.37, sa.4, ss.174-178, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 37 Sayı: 4
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/j.ijbiomac.2005.10.004
  • Dergi Adı: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.174-178
  • Anahtar Kelimeler: glucose oxidase, enzyme immobilization, electrochemistry, glucose determination, orange juice, INVERTASE
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Glucose oxidase was immobilized in conducting copolymers of three different types of poly(methyl methacrylate-co-thienyl methacrylate). Immobilization of enzyme was carried out by the entrapment in conducting polymers during electrochemical polymerization of pyrrole on the copolymer electrodes. Maximum reaction rate, Michaelis-Menten constants, temperature, pH and operational stabilities were determined for immobilized enzyme. The amount of glucose in orange juices of Turkey was investigated by using enzyme electrodes. (c) 2005 Elsevier B.V. All rights reserved.