Humanized brain organoids-on-chip integrated with sensors for screening neuronal activity and neurotoxicity


Saglam-Metiner P., YILDIRIM E., Dincer C., Basak O., Yesil-Celiktas O.

Microchimica Acta, cilt.191, sa.1, 2024 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 191 Sayı: 1
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1007/s00604-023-06165-4
  • Dergi Adı: Microchimica Acta
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Food Science & Technology Abstracts, Metadex, Pollution Abstracts, Civil Engineering Abstracts
  • Anahtar Kelimeler: Biocomputing, Bioengineering, Brain organoids, Microfluidics, Organoid-on-a-chip, Sensors
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

The complex structure and function of the human central nervous system that develops from the neural tube made in vitro modeling quite challenging until the discovery of brain organoids. Human-induced pluripotent stem cells–derived brain organoids offer recapitulation of the features of early human neurodevelopment in vitro, including the generation, proliferation, and differentiation into mature neurons and micro-macroglial cells, as well as the complex interactions among these diverse cell types of the developing brain. Recent advancements in brain organoids, microfluidic systems, real-time sensing technologies, and their cutting-edge integrated use provide excellent models and tools for emulation of fundamental neurodevelopmental processes, the pathology of neurological disorders, personalized transplantation therapy, and high-throughput neurotoxicity testing by bridging the gap between two-dimensional models and the complex three-dimensional environment in vivo. In this review, we summarize how bioengineering approaches are applied to mitigate the limitations of brain organoids for biomedical and clinical research. We further provide an extensive overview and future perspectives of the humanized brain organoids-on-chip platforms with integrated sensors toward brain organoid intelligence and biocomputing studies. Such approaches might pave the way for increasing approvable clinical applications by solving their current limitations. Graphical Abstract: [Figure not available: see fulltext.]