Sub-cellular distribution of two salt-induced peptides in roots of Oryza sativa L. var Nonabokra

Naqvi S. M. S., Raza S. Q., Hyder M. Z., Ozalp C. V., Oktem H. A., Yucel M.

AFRICAN JOURNAL OF BIOTECHNOLOGY, cilt.8, sa.18, ss.4613-4617, 2009 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 8 Sayı: 18
  • Basım Tarihi: 2009
  • Dergi Adı: AFRICAN JOURNAL OF BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.4613-4617
  • Anahtar Kelimeler: 2-D electrophoresis, fractionation, salt induced proteins, sub-cellular localization, 2-DIMENSIONAL ELECTROPHORESIS, BARLEY ROOTS, STRESS, RICE, PROTEINS, EXPRESSION, WHEAT, SEEDLINGS, SALINITY, CULTIVAR
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Living systems respond to changing environment by changing gene expression. In addition to changes in the level of expression of existing proteins, some new proteins may be synthesized. Such proteins not only can serve as markers, but may also play important role in adjusting to that specific environment. Targeting of these proteins to specific location within or outside the cell may also yield important clues to their function. In such a pursuit, ten days old seedlings of a relatively salt tolerant rice variety grown on MS medium were treated with 2% sodium chloride for three days. Root were harvested, ground, and fractionated in to sub-cellular components. Microsomes were further fractionated into constituent membranes. Proteins from these fractions were analyzed by SDS-PAGE and/or two dimensional separations. Results show induction of two new polypeptides which were present in total protein extract and soluble fractions. All other fractions failed to reveal induction of any new peptide under experimental conditions used.