Silver nanoparticles-tragacanth gel as a green membrane for effective extraction and determination of capecitabine

Hajizadeh S., Farhadi K., Molaei R., Forough M.

Journal of Separation Science, cilt.43, sa.13, ss.2666-2674, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43 Sayı: 13
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1002/jssc.202000251
  • Dergi Adı: Journal of Separation Science
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, BIOSIS, CAB Abstracts, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, INSPEC, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.2666-2674
  • Anahtar Kelimeler: capecitabine, electromembrane extraction, silver nanoparticles, tragacanth gel, ELECTROMEMBRANE EXTRACTION, LIQUID-CHROMATOGRAPHY, CAPILLARY-ELECTROPHORESIS, HUMAN PLASMA, METABOLITES, 5-FLUOROURACIL, QUANTIFICATION, CHEMOTHERAPY, DRUGS
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimA novel eco-friendly and effective electromembrane extraction method combining high-performance liquid chromatography with UV detection was developed for the enrichment and determination of capecitabine. Tragacanth-silver nanoparticles conjugated gel was prepared by dissolving the tragacanth powder in synthesized silver nanoparticles solution and was used as a green membrane in electromembrane extraction. The porosity and presence of silver nanoparticles in the gel were characterized by field emission scanning electron microscopy. This new electromembrane extraction approach uses neither organic solvent nor carrier agents to extract the target analyte. The best electromembrane extraction efficiency was obtained by using 4.0 mm membrane gel thickness containing 2.5% w/v of tragacanth gum, donor phase pH = 5.0, acceptor phase pH = 3.0, applied voltage 50 V, extraction time 20 min, and agitation rate 500 rpm. During method validation under the optimized conditions, good linearity dynamic range between 1 and 500 ng/mL with the coefficient of determination (R2) = 0.998 was obtained. Limit of detection and Limit of quantitation were estimated to be 0.84 and 1.0 ng/mL, respectively. Finally, the applicability of this method in real samples was confirmed by an acceptable performance in extraction and determination of capecitabine in human plasma samples.