A benzimidazole-based conducting polymer and a PMMA-clay nanocomposite containing biosensor platform for glucose sensing


EMRE F. B. , Kesik M., Kanik F. E. , Akpinar H. Z. , Aslan-Gurel E., Rossi R. M. , ...More

SYNTHETIC METALS, vol.207, pp.102-109, 2015 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 207
  • Publication Date: 2015
  • Doi Number: 10.1016/j.synthmet.2015.06.015
  • Title of Journal : SYNTHETIC METALS
  • Page Numbers: pp.102-109
  • Keywords: Conducting polymer, Polymer clay nanocomposite, Laponite, Glucose biosensor, Immobilization platform, NYLON 6-CLAY HYBRID, ELECTROCHEMICAL POLYMERIZATION, BIOMOLECULE IMMOBILIZATION, MODIFIED ELECTRODES, PI-STACKING, LAPONITE, FILMS, POLYPYRROLE, EFFICIENCY, OXIDASE

Abstract

Development of materials composed of polymer-clay nanocomposites (PCN) and conducting polymers attracts great interest and preferred in various applications. Hereby, polymethylmethacrylate (PMMA) layered silicate nanocomposites were prepared by in-situ suspension polymerization by grafting PMMA with laponite using a suitable grafting agent. The properties of the as-synthesized PCN materials are characterized by differential scanning calorimetry (DSC), thermal gravimetry analysis (TGA) and gel permeation chromatography (GPC). A conducting polymer; poly(4-(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-y1)-7-(2,3-dihydrothieno[3,4-b][1,4]dioxin-7-y1)-2-benzyl-1H-benzo[d]imidazole) (poly(BIPE)) and a PMMA-clay nanocomposite with 2-(methacryloyloxy) ethyltrimethylammonium chloride (MTMA) modifier were examined as a platform for biomolecule deposition. Glucose oxidase (GOx, beta-D-glucose: oxygen-1-oxidoreductase, EC 1.1.3.4) was chosen as the model enzyme to prepare a scaffold for glucose sensing. Three different sensing strategies; PCN/GOx, poly(BIPE)/GOx and PCN/poly(BIPE)/GOx were analyzed and their biosensor performances were discussed. Surface morphology of the modified electrodes was characterized by scanning electron microscopy (SEM) technique. Electrochemical responses of the enzyme electrodes were monitored at -0.7 V vs. Ag reference electrode by monitoring oxygen consumption in the presence of glucose. After optimum conditions were determined, kinetic and analytical parameters; K-M(aPP), I-max, LOD and sensitivity were investigated for each sensing platform. (C) 2015 Elsevier B.V. All rights reserved.