A novel promising biomolecule immobilization matrix: Synthesis of functional benzimidazole containing conducting polymer and its biosensor applications

Uzun S. D., Unlu N. A., Sendur M., Kanik F. E., TİMUR S., TOPPARE L. K.

COLLOIDS AND SURFACES B-BIOINTERFACES, cilt.112, ss.74-80, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 112
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1016/j.colsurfb.2013.07.049
  • Dergi Adı: COLLOIDS AND SURFACES B-BIOINTERFACES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.74-80
  • Anahtar Kelimeler: Conducting polymer, Biosensor, Enzyme immobilization, Low detection limit, Glucose level, HYDROGEN-PEROXIDE, GLUCOSE-OXIDASE, COVALENT IMMOBILIZATION, ELECTRODE, FILM, CELL, NANOSTRUCTURES, NANOPARTICLES, DEVICES, SENSORS
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

In order to construct a robust covalent binding between biomolecule and immobilization platform in biosensor preparation, a novel functional monomer 4-(4,7-di(thiophen-2-yl)-1H-benzo[d]imidazol-2yl)benzaldehyde (BIBA) was designed and successfully synthesized. After electropolymerization of this monomer, electrochemical and spectroelectrochemical properties were investigated in detail. To fabricate the desired biosensor, glucose oxidase (GOx) was immobilized as a model enzyme on the polymer coated graphite electrode with the help of glutaraldehyde (GA). During the immobilization step, an imine bond was formed between the free amino groups of enzyme and aldehyde group of polymer. The surface characterization and morphology were investigated to confirm bioconjugation by X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM) at each step of biosensor fabrication. The optimized biosensor shows good linearity between 0.02 mM and 1.20 mM and a low limit of detection (LOD) of 2.29 mu M. Kinetic parameters K-m(app) and I-max were determined as 0.94 mM and 10.91 mu A, respectively. The biosensor was tested for human blood serum samples. (C) 2013 Elsevier B.V. All rights reserved.