IMMOBILIZATION OF ALPHA-AMYLASE INTO PHOTOGRAPHIC GELATIN BY CHEMICAL CROSS-LINKING


BAYRAMOGLU Z., AKBULUT U., SUNGUR S.

BIOMATERIALS, cilt.13, sa.10, ss.704-708, 1992 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13 Sayı: 10
  • Basım Tarihi: 1992
  • Doi Numarası: 10.1016/0142-9612(92)90131-7
  • Dergi Adı: BIOMATERIALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.704-708
  • Anahtar Kelimeler: IMMOBILIZATION, ENZYMES, GELATIN, CROSS-LINKING
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Alpha-amylase was immobilized into photographic gelatin by chemical cross-linking with chromium (III) acetate and chromium (III) sulphate. Cellulose triacetate film strips, enabled simple handling when coated with an alpha-amylase-gelatin mixture, accomplishing a high degree of durability during consecutive immersions into reaction media. The optimum conditions for pH, substrate concentration, temperature, incubation time and storing conditions of free and immobilized alpha-amylase were determined. The effect of use number on activity and the effect of resting on reuse number were also determined. Photographic gelatin was found to be a very efficient natural polymer, due to its extraordinary diffusion characteristics for immobilization as a carrier.