Decreased bacteria activity on Si3N4 surfaces compared with PEEK or titanium


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Gorth D. J., Puckett S., Ercan B., Webster T. J., Rahaman M., Bal B. S.

INTERNATIONAL JOURNAL OF NANOMEDICINE, cilt.7, ss.4829-4840, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 7
  • Basım Tarihi: 2012
  • Doi Numarası: 10.2147/ijn.s35190
  • Dergi Adı: INTERNATIONAL JOURNAL OF NANOMEDICINE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.4829-4840
  • Anahtar Kelimeler: silicon nitride, nanostructure, anti-infective, biofilm, protein adsorption, ADHESION IN-VITRO, OSTEOBLAST FUNCTIONS, NANOPHASE CERAMICS, STAINLESS-STEEL, ANTIBACTERIAL ACTIVITY, MICROBIAL ADHESION, ANODIZED TITANIUM, EXTERNAL FIXATION, CELL ATTACHMENT, SILICON-NITRIDE
  • Orta Doğu Teknik Üniversitesi Adresli: Hayır

Özet

A significant need exists for orthopedic implants that can intrinsically resist bacterial colonization. In this study, three biomaterials that are used in spinal implants - titanium (Ti), polyether-ether-ketone (PEEK), and silicon nitride (Si3N4) - were tested to understand their respective susceptibility to bacterial infection with Staphylococcus epidermidis, Staphlococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus. Specifically, the surface - chemistry, wettability, and nanostructured topography of respective biomaterials, and the effects on bacterial biofilm formation, colonization, and growth were investigated. Ti and PEEK were received with as-machined surfaces; both materials are hydrophobic, with net negative surface charges. Two surface finishes of Si3N4 were examined: as-fired and polished. In contrast to Ti and PEEK, the surface of Si3N4 is hydrophilic, with a net positive charge. A decreased biofilm formation was found, as well as fewer live bacteria on both the as- fired and polished Si3N4. These differences may reflect differential surface chemistry and surface nanostructure properties between the biomaterials tested. Because protein adsorption on material surfaces affects bacterial adhesion, the adsorption of fibronectin, vitronectin, and laminin on Ti, PEEK, and Si3N4 were also examined. Significantly greater amounts of these proteins adhered to Si3N4 than to Ti or PEEK. The findings of this study suggest that surface properties of biomaterials lead to differential adsorption of physiologic proteins, and that this phenomenon could explain the observed in-vitro differences in bacterial affinity for the respective biomaterials. Intrinsic biomaterial properties as they relate to resistance to bacterial colonization may reflect a novel strategy toward designing future orthopedic implants.