Cloning and expression of trehalose-6-phosphate synthase 1 from Rhizopus oryzae


Uyar E. O., Yucel M., HAMAMCI H.

JOURNAL OF BASIC MICROBIOLOGY, vol.56, no.5, pp.459-468, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 56 Issue: 5
  • Publication Date: 2016
  • Doi Number: 10.1002/jobm.201500425
  • Journal Name: JOURNAL OF BASIC MICROBIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.459-468
  • Keywords: Cloning, Rhizopus oryzae, Trehalose, Trehalose-6-phosphate synthase, TREHALOSE 6-PHOSPHATE SYNTHASE, SACCHAROMYCES-CEREVISIAE, SELAGINELLA-LEPIDOPHYLLA, MOLECULAR CHARACTERIZATION, RESERVE CARBOHYDRATE, ESCHERICHIA-COLI, YEAST, GENE, STRESS, COMPLEMENTATION
  • Middle East Technical University Affiliated: Yes

Abstract

Trehalose is a reducing disaccharide acting as a protectant against environmental stresses in many organisms. In fungi, Trehalose-6-phosphate synthase 1 (TPS1) plays a key role in the biosynthesis of trehalose. In this study, a full-length cDNA from Rhizopus oryzae encoding TPS1 (designated as RoTPS1) was isolated. The RoTPS1 cDNA is composed of 2505 nucleotides and encodes a protein of 834 amino acids with a molecular mass of 97.8kDa. The amino acid sequence of RoTPS1 has a relatively high homology with the TPS1s in several other filamentous fungi. RoTPS1 was cloned into Saccharomyces cerevisiae and secretively expressed.