Study of zeolite influence on analytical characteristics of urea biosensor based on ion-selective field-effect transistors

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Shelyakina M. K., Soldatkin O. O., Arkhypova V. M., Kasap B. O., AKATA KURÇ B., Dzyadevych S. V.

NANOSCALE RESEARCH LETTERS, cilt.9, 2014 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 9
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1186/1556-276x-9-124
  • Dergi Adı: NANOSCALE RESEARCH LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Biosensor, Urease, Silicalite, Zeolite, Nano beta, Nano L, pH-sensitive field-effect transistor, BETA
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

A possibility of the creation of potentiometric biosensor by adsorption of enzyme urease on zeolite was investigated. Several variants of zeolites (nano beta, calcinated nano beta, silicalite, and nano L) were chosen for experiments. The surface of pH-sensitive field-effect transistors was modified with particles of zeolites, and then the enzyme was adsorbed. As a control, we used the method of enzyme immobilization in glutaraldehyde vapour (without zeolites). It was shown that all used zeolites can serve as adsorbents (with different effectiveness). The biosensors obtained by urease adsorption on zeolites were characterized by good analytical parameters (signal reproducibility, linear range, detection limit and the minimal drift factor of a baseline). In this work, it was shown that modification of the surface of pH-sensitive field-effect transistors with zeolites can improve some characteristics of biosensors.