Agropyron cristatum (L.) Gaertn. is represented by 2 subspecies in Turkey, namely subsp. incanum (Nabelek) Melderis and subsp. pectinatum (M.Bieb.) Tzvelev. This study examines the taxonomic significance of the leaf properties of the taxa of Agropyron Gaertn., comprising diagnostic anatomical features obtained from leaf transverse sections as well as leaf surface micromorphology. Results of the study show that most of the characters, such as the shape of the leaf blades; the size and number of stomata; the density, arrangement, and length of macro hairs; and the presence or absence of midrib and bulliform cells are different in the studied samples of Agropyron cristatum.