Arachidonic acid modulation of [3H]naloxone specific binding to rat brain opioid receptors

ÖKTEM H. A., Apaydin S.

Neurobiology, vol.6, no.3, pp.323-332, 1998 (Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 6 Issue: 3
  • Publication Date: 1998
  • Journal Name: Neurobiology
  • Journal Indexes: Scopus
  • Page Numbers: pp.323-332
  • Keywords: [3H]naloxone, Arachidonic acid, Mu- and delta-opioid receptors, Rat brain opioid receptors
  • Middle East Technical University Affiliated: Yes

Abstract

Arachidonic acid effect on binding of [3H]naloxone to rat brain membranes, were studied at opioid receptor subtype level. Arachidonic acid inhibited opioid receptor binding in a dose-dependent manner, both in the presence and absence of sodium. With blockage experiments it was shown that delta-opioid receptors were modulated by arachidonic acid to a greater extent than that of mu-opioid receptors. On the other hand, there was no significant difference in terms of IC50 values for arachidonic acid inhibiton of [3H]naloxone binding at agonist and antagonist configuration of the receptor subtypes.