Elucidating the biosurfactant potential of Bacillus strains: A study of structure and function in food applications

Gharsallah H., Guerin C., Eddehech A., Boufi S., ÖZTOP H. M., Balti R., ...Daha Fazla

International Journal of Food Science and Technology, cilt.60, sa.1, 2025 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 60 Sayı: 1
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1093/ijfood/vvaf116
  • Dergi Adı: International Journal of Food Science and Technology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Compendex, Food Science & Technology Abstracts, INSPEC, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Bacillus, biosurfactant, functional properties, lipopeptides, Liquid chromatography-tandem mass spectrometry
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

This study explores the isolation and characterisation of biosurfactant-producing Bacillus strains. Four β-hemolytic isolates Bacillus velezensis (H6), Bacillus subtilis (S15 and S4), and Bacillus cereus (S32) were identified as potential biosurfactant producers. Among them, strain H6 showed the highest activity, reducing surface tension by 35 mN/m. This surface activity was further supported by FTIR analysis, which confirmed the lipopeptide nature of the compounds. In addition, thermogravimetric analysis indicated the thermal stability up to 140-190 °C. The biosurfactants also exhibited low critical micelle concentration values (0.3-0.5 g/L) outperformed sodium dodecyl sulphate and confirming their strong surface-active potential. Liquid chromatography-tandem mass spectrometry revealed diverse lipopeptide families, including surfactins, iturins, and fengycins, with strain H6 producing the highest levels. Overall, the biosurfactants displayed excellent functional properties, achieving up to 90% emulsification and foaming activity, which highlights their applicability in industrial sectors, particularly in food processing.