Amperometric biosensor for cholesterol based on novel nanocomposite array gold nanoparticles/acetone-extracted propolis/multiwall carbon nanotubes/gold


Molaei R., Sabzi R. E. , Farhadi K., Kheiri F., Forough M.

MICRO & NANO LETTERS, vol.9, no.2, pp.100-104, 2014 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 9 Issue: 2
  • Publication Date: 2014
  • Doi Number: 10.1049/mnl.2013.0664
  • Journal Name: MICRO & NANO LETTERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.100-104
  • Keywords: amperometric sensors, biochemistry, biosensors, carbon nanotubes, electrochemical electrodes, enzymes, gold, hydrogen compounds, molecular biophysics, nanobiotechnology, nanocomposites, nanoparticles, voltammetry (chemical analysis), nanosensors, amperometric biosensor, cholesterol oxidase, nanocomposite array, gold nanoparticles, acetone-extracted propolis, multiwall carbon nanotubes, electrochemical electrodes, hydrogen peroxide detection, cyclic voltammetry, enzymatic biosensor, sensitivity, cost effective detection, electroactive species, ascorbic acid, glucose, Au, H2O2, COMPOSITE FILM, SILVER NANOPARTICLES, PROPOLIS, ESTERASE, OXIDASE, ELECTRODE, CHITOSAN
  • Middle East Technical University Affiliated: No

Abstract

In this reported work, the electrochemical behaviour of gold electrodes modified with gold nanoparticles, acetone-extracted propolis, multiwall carbon nanotubes and cholesterol oxidase was established for the detection of hydrogen peroxide by using cyclic voltammetry and amperometric techniques. The obtained results confirmed that the current enzymatic biosensor exhibits a fast, highly sensitive, and cost-effective detection of cholesterol. Cholesterol in the concentration range of 0.15-0.55 mmol l(-1) was determined with a detection limit of 4.9 x 10(-5) mol l(-1) by the amperometric method, and the sensitivity of the proposed method was found to be 17.38 mu A/mmol l(-1). Normal electroactive species such as ascorbic acid and glucose in the presence of the constant concentration of cholesterol in the samples do not interfere with the determination.