Flow Cytometric and Immunohistochemical Follow-Up of Spermatogonial Lineage Commitment

Önen S., Gizer M., KORKUSUZ P.

Methods in molecular biology (Clifton, N.J.), cilt.2849, ss.239-251, 2024 (Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 2849
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1007/7651_2023_506
  • Dergi Adı: Methods in molecular biology (Clifton, N.J.)
  • Derginin Tarandığı İndeksler: Scopus, Biotechnology Research Abstracts, CAB Abstracts, MEDLINE
  • Sayfa Sayıları: ss.239-251
  • Anahtar Kelimeler: Flow cytometry, Immune labeling, Immunohistochemistry, Spermatogonial lineage commitment, Spermatogonial stem cells
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Flow cytometry and immunohistochemistry techniques both determine the target protein by immunolabeling. Flow cytometric analysis quantifies total number of fluorescent labeled cells and qualify sup-populations according to size and granularity. Immunohistochemistry is able to map immune-labeled cells and extracellular matrix components under light and electron microscope by enzyme or fluorescent molecules. Real-time identification, in-time classification, and final plotting of spermatogonial lineage are of crucial importance for monitoring the fertility potential of spermatogonial stem cell microenvironment and predicting progress of spermatogenesis. Here we define the evaluation of mouse male germ cell microenvironment at single cell and whole tissue section levels by using flow cytometric and immunohistochemical approaches.