Studying Tunicata WBR Using Botrylloides anceps

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Karahan A., Öztürk E., Temiz B., Blanchoud S.

in: Whole-Body Regeneration Methods and Protocols, Simon Blanchoud,Brigitte Galliot, Editor, Springer, London/Berlin , New York, pp.311-335, 2022

  • Publication Type: Book Chapter / Chapter Research Book
  • Publication Date: 2022
  • Publisher: Springer, London/Berlin 
  • City: New York
  • Page Numbers: pp.311-335
  • Editors: Simon Blanchoud,Brigitte Galliot, Editor
  • Middle East Technical University Affiliated: Yes


Tunicates are marine filter-feeding invertebrates that can be found worldwide and which are the closest phylogenetic group to the vertebrates (Craniata). Of particular interest, colonial tunicates are the only known chordates that can undergo Whole-Body Regeneration (WBR) via vascular budding. In Botrylloides anceps, a fully functional adult regenerates from a fragment of the vascular system in around 2 weeks after amputation. In this chapter, we present protocols to collect B. anceps colonies, confirm their species, breed them in the lab, monitor WBR and perform histological staining on cryosections.