Assessment and optimization of xylanase production using co-cultures of Bacillus subtilis and Kluyveromyces marxianus
3 BIOTECH, cilt.8, sa.7, 2018 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 8 Sayı: 7
- Basım Tarihi: 2018
- Doi Numarası: 10.1007/s13205-018-1315-y
- Dergi Adı: 3 BIOTECH
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Anahtar Kelimeler: Xylanase, Optimization, Co-culture, Submerged fermentation, Bacillus subtilis, Kluyveromyces marxianus, CELLULASE-FREE XYLANASE, ASPERGILLUS-NIGER, SUBMERGED FERMENTATION, MIXED CULTURE, PURIFICATION, PARAMETERS, ENZYMES
- Orta Doğu Teknik Üniversitesi Adresli: Evet
Özet
Co-cultures of Bacillus subtilis and Kluyveromyces marxianus were investigated in submerged fermentation for xylanase production at shake-flask scale. Xylanase production markedly increased when arabinose, xylose, or hazelnut shells were used as the single carbon source. Maximal xylanase of 49.5 IU/mL was achieved with 4% B. subtilis, 4% K. marxianus, 40% solid load of hazelnut shells, and pH 7.0. Overall, xylanase was enhanced by 4.4-fold compared to initial un-optimized monoculture production and 2.8-fold compared to initial un-optimized co-cultured production, after optimization by response surface method.