Assessment and optimization of xylanase production using co-cultures of Bacillus subtilis and Kluyveromyces marxianus


Yardimci G. O. , ÇEKMECELİOĞLU D.

3 BIOTECH, vol.8, no.7, 2018 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 8 Issue: 7
  • Publication Date: 2018
  • Doi Number: 10.1007/s13205-018-1315-y
  • Title of Journal : 3 BIOTECH
  • Keywords: Xylanase, Optimization, Co-culture, Submerged fermentation, Bacillus subtilis, Kluyveromyces marxianus, CELLULASE-FREE XYLANASE, ASPERGILLUS-NIGER, SUBMERGED FERMENTATION, MIXED CULTURE, PURIFICATION, PARAMETERS, ENZYMES

Abstract

Co-cultures of Bacillus subtilis and Kluyveromyces marxianus were investigated in submerged fermentation for xylanase production at shake-flask scale. Xylanase production markedly increased when arabinose, xylose, or hazelnut shells were used as the single carbon source. Maximal xylanase of 49.5 IU/mL was achieved with 4% B. subtilis, 4% K. marxianus, 40% solid load of hazelnut shells, and pH 7.0. Overall, xylanase was enhanced by 4.4-fold compared to initial un-optimized monoculture production and 2.8-fold compared to initial un-optimized co-cultured production, after optimization by response surface method.