3rd International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2020), Ankara, Turkey, 19 - 20 March 2020, pp.134
Poly(2,5-di(furan-2-yl)thiazolo[5,4-d]thiazol) polymer was coated over a platinum electrode and new enzyme electrodes
were produced that are not found in the literature. The formation of polymerization was shown by cyclic voltammetry.
Kinetic parameters of immobilized polyphenol oxidase enzyme were determined. Vmax and Km were obtained as 0.028 ±
0.001 umol min1- electrode1- and 669.68 ± 64.73 mM respectively. The effect of reaction conditions over enzyme activity
were examined. The pH and temperature at which immobilized polyphenol oxidase enzyme showed the highest activity
were found as pH 7.5 and 45 ℃. Amount of enzyme and gluteraldehyde immobilized to the electrodes were changed and in
the examination, it was detected that it is sufficient to add 2x6 uL enzyme and 1x6 uL gluteraldehyde to the electrode. The
activity of Pt/PTTzFr/PPO electrode was followed by 50 consequential measurements. It has been seen after 50
measurements that immobilized enzyme activity is 70%. As a result of shelf life examinations, it was determined that
Pt/PTTzFr/PPO electrode lost 30% of its initial activity at the end of the 50th day and the remaining activity was 70%.
Calibration graph was plotted for Pt/PTTzFr/PPO electrode (y = 0.0139x + 0.0479). LOQ value was calculated as 7.827 mg
mL-1 and the working interval was found as 1.0 – 90.0 mg mL1-. In total phenolic material analyses made with this
electrode, the results found in waste water samples have also been confirmed by Folin-Ciocalteau method.