Utilization of pretreated molasses for serine alkaline protease production with recombinant Bacillus species

Calik G., Pehlivan N., Kalender N., Ozdamar T., Calik P.

CHEMICAL ENGINEERING COMMUNICATIONS, vol.190, pp.630-644, 2003 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 190
  • Publication Date: 2003
  • Doi Number: 10.1080/00986440302125
  • Page Numbers: pp.630-644


Recombinant Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis , and Bacillus licheniformis were used for the production of serine alkaline protease (SAP) utilizing chemically and/or physically pretreated molasses. The highest enzyme activity was obtained with r- Bacillus subtilis , with the complex medium involving physically treated molasses having 20 kg m(-3) initial sucrose concentration in small-scale, agitation- and heating rate-controlled bioreactors at t=63 h. Effects of oxygen transfer were investigated in 3.5 dm(3) laboratory bioreactors under two different agitation rates with r-B. subtilis . Increase in the oxygen transfer rate increased the observed activity and caused the cultivation time of maximum activity shift to the earlier stages of the fermentation. At Q/V=0.5 vvm and N=750 min(-1) , SAP activity reached 2250 U cm(-3) at t=36 h.