Microbial growth and attachment of Salmonella and enterohemorrhagic and enteroaggregative Escherichia coli strains on cress microgreens grown in peat soil system

Namli S., Samut H., Soyer Y.

BRITISH FOOD JOURNAL, cilt.124, sa.11, ss.3765-3782, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 124 Sayı: 11
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1108/bfj-03-2021-0269
  • Dergi Adı: BRITISH FOOD JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, ABI/INFORM, Aerospace Database, Agricultural & Environmental Science Database, CAB Abstracts, Communication Abstracts, Food Science & Technology Abstracts, Hospitality & Tourism Complete, INSPEC, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.3765-3782
  • Anahtar Kelimeler: Salmonella, E, coli, Biofilm, Attachment, Seed contamination, Fresh produce, Cress, Microgreen, Irrigation, CONTAMINATED MANURE COMPOSTS, HEMOLYTIC-UREMIC SYNDROME, HUMAN ENTERIC PATHOGENS, LEAFY GREEN VEGETABLES, CLIMATE-CHANGE, O157H7, SURVIVAL, LEAVES, PERSISTENCE, PREHARVEST
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Purpose This study aimed to investigate how enteric pathogens and their biofilm populations on fresh produce survive according to time that contamination has occurred on leaves and contamination route: seed irrigation water. Design/methodology/approach Cress was contaminated in two different ways: contamination of seeds and irrigation water with 8-log MPN/mL bacterial load, Salmonella Newport, Escherichia coli O157:H7, O104:H4 or O78:H2. While contaminated seeds were cultivated for seed contamination, contaminated irrigation was applied at the end of each week to separate groups of samples obtained from cultivated surface-sterile seeds to understand how long these pathogens could survive until harvest. Findings The results indicated these pathogens survived until harvest, and formed biofilms on cress leaves grown using both contaminated seeds and irrigation water. No significant difference was observed among populations of Salmonella and E. coli groups in terms of survival (similar to 4.5-6.0 log MPN/g) and biofilm formation (similar to 4.4-5.7 log MPN/g) for contamination by seed. Also, SEM images revealed biofilm-like structures, the proofs of the attachment of these pathogens on leaf surfaces. Originality/value From our knowledge this is the first study focusing on the survival and biofilm formation of one Salmonella serotype (Newport) and three E. coli serotypes (O157:H7, O104:H4, and O78:H2), representing enterohemorrhagic and enteroaggregative E. coli pathogenic subgroups, under the same irrigation and growth schemes. Furthermore, this study mimics the contamination of seeds and irrigation water with sewage or wastewater and may shed light on contamination of fresh produce grown using poor wastewater treatment.