© 2020 Elsevier LtdThe aim of the present study is to investigate the mediating roles of driving skills in relationship between organizational safety strategies and driver behaviours among driving instructors. Driving skills consist of perceptual-motor skills and safety skills. Driver behaviours are investigated under four factors: violations, errors, lapses, and positive driver behaviours. Participants were 132 driving instructors (108 male and 24 female). In order to measure organizational safety strategies, Organizational Safety Strategies Scale (OSSS) was developed for driving schools. Results of the principal component analyses yielded one-factor solution for OSSS. In order to test the indirect effects of organizational safety strategies on driver behaviours through driving skills, multiple mediation analyses were conducted by entering age and annual mileage as the control variables. As organizational safety strategies were stronger, driving instructors had higher levels of perceptual-motor skills, which resulted in higher violations. On the other hand, as organizational safety strategies were stronger, driving instructors had higher levels of safety skills, which resulted in less violations and lapses. It can be inferred that; organizational stronger safety strategies might have negative influences on road safety through higher perceptual-motor skills; whereas there can be positive influences on road safety through higher safety skills. In addition, both skills are related to organizational safety strategies. Hence, driving schools should consider the asymmetric relationship between perceptual-motor skills and safety skills while improving their safety strategies to decrease violations and lapses. Organizations might also develop interventions to balance the stated skills to increase road safety.