Immobilization of cholesterol oxidase in a conducting copolymer of thiophene-3-yl acetic acid cholesteryl ester with pyrrole

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Cirpan A., ALKAN S., TOPPARE L. K., Cianga I., YAGCI Y.

DESIGNED MONOMERS AND POLYMERS, cilt.6, sa.3, ss.237-243, 2003 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 6 Sayı: 3
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1163/156855503768336252
  • Dergi Adı: DESIGNED MONOMERS AND POLYMERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.237-243
  • Anahtar Kelimeler: cholesterol oxidase, immobilization, conducting polymer, polypyrrole, electrochemical polymerization, IN-SITU PREPARATION, AMPEROMETRIC DETERMINATION, BIOSENSOR, ELECTROPOLYMERIZATION, PEROXIDASE, SERUM
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Cholesterol oxidase has been immobilized in conducting copolymers of thiophene-3-yl acetic acid cholesteryl ester with pyrrole (CM/PPy) and polypyrrole (PPy) via electropolymerization. p-Toluene sulphonic acid was used as the supporting electrolyte. Kinetic parameters (V-max and K-m) and operational stability of enzyme electrodes were investigated. Surface morphology of the films was examined by scanning electron microscope.