Immobilization of cholesterol oxidase in a conducting copolymer of thiophene-3-yl acetic acid cholesteryl ester with pyrrole


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Cirpan A. , ALKAN S., TOPPARE L. K. , Cianga I., YAGCI Y.

DESIGNED MONOMERS AND POLYMERS, cilt.6, ss.237-243, 2003 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 6 Konu: 3
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1163/156855503768336252
  • Dergi Adı: DESIGNED MONOMERS AND POLYMERS
  • Sayfa Sayıları: ss.237-243

Özet

Cholesterol oxidase has been immobilized in conducting copolymers of thiophene-3-yl acetic acid cholesteryl ester with pyrrole (CM/PPy) and polypyrrole (PPy) via electropolymerization. p-Toluene sulphonic acid was used as the supporting electrolyte. Kinetic parameters (V-max and K-m) and operational stability of enzyme electrodes were investigated. Surface morphology of the films was examined by scanning electron microscope.