Immobilization of cholesterol oxidase in a conducting copolymer of thiophene-3-yl acetic acid cholesteryl ester with pyrrole


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Cirpan A., ALKAN S., TOPPARE L. K. , Cianga I., YAGCI Y.

DESIGNED MONOMERS AND POLYMERS, vol.6, no.3, pp.237-243, 2003 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 6 Issue: 3
  • Publication Date: 2003
  • Doi Number: 10.1163/156855503768336252
  • Journal Name: DESIGNED MONOMERS AND POLYMERS
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.237-243
  • Keywords: cholesterol oxidase, immobilization, conducting polymer, polypyrrole, electrochemical polymerization, IN-SITU PREPARATION, AMPEROMETRIC DETERMINATION, BIOSENSOR, ELECTROPOLYMERIZATION, PEROXIDASE, SERUM

Abstract

Cholesterol oxidase has been immobilized in conducting copolymers of thiophene-3-yl acetic acid cholesteryl ester with pyrrole (CM/PPy) and polypyrrole (PPy) via electropolymerization. p-Toluene sulphonic acid was used as the supporting electrolyte. Kinetic parameters (V-max and K-m) and operational stability of enzyme electrodes were investigated. Surface morphology of the films was examined by scanning electron microscope.