Comparison of chemical fractionation method and H-1-NMR spectroscopy in measuring the monomer block distribution of algal alginates


Moral C. K. , Doğan Ö. , Sanin F. D.

JOURNAL OF POLYMER ENGINEERING, cilt.33, ss.239-246, 2013 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 33
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1515/polyeng-2012-0066
  • Dergi Adı: JOURNAL OF POLYMER ENGINEERING
  • Sayfa Sayısı: ss.239-246

Özet

Alginates are composed of beta-D-mannuronic acid (M) and alpha-L-guluronic acid (G). In this study, block distributions of alginate samples were measured by a chemical fractionation method and H-1-NMR spectroscopy, to compare the usefulness of the two methods. Block distribution results obtained from the two methods were found to be statistically different from each other; the chemical fractionation method gave consistently lower estimates for the amount of homopolymeric blocks. The repeatability of the results was also lower in the chemical fractionation method. By contrast, H-1-NMR spectroscopy was found to be a reproducible and a faster method for determining the distribution of monomeric blocks in alginates.