Development of a novel biosensor based on a conducting polymer


SÖYLEMEZ S., Kanik F. E. , Ileri M., Hacioglu S. O. , TOPPARE L. K.

TALANTA, vol.118, pp.84-89, 2014 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 118
  • Publication Date: 2014
  • Doi Number: 10.1016/j.talanta.2013.10.007
  • Title of Journal : TALANTA
  • Page Numbers: pp.84-89

Abstract

A new type of amperometric cholesterol biosensor was fabricated to improve the biosensor characteristics such as sensitivity and reliability. For this purpose, a novel immobilization matrix 2-(4-fluorophenyl)-4,7-di(thiophene-2-yl)-1H-benzo[d]imidazole (BIPF) was electrochemically deposited on a graphite electrode and used as a matrix for the immobilization of cholesterol oxidase (ChOx). Due to strong pi-pi stacking of aromatic groups in the structures of polymer backbone and enzyme molecule, one can easily achieve a sensitive and reliable biosensor without using any membrane or covalent bond formation between the enzyme molecules and polymer surface. Moreover, through pendant fluorine group of the polymer, H-bond formation between with enzyme molecules and polymer was generated. Cholesterol was used as the substrate and amperometric response was measured in correlation with cholesterol amount, at -0.7 V vs. Ag/AgCl in phosphate buffer (pH 7.0). Consequently, optimum conditions for this constructed biosensor were determined. K(M)app, I-max, LOD and sensitivity values were investigated and calculated as 4.0 nM, 2.27 mu A, 0.404 mu M and 1.47 mA/mM cm(2), respectively. A novel and accurate cholesterol biosensor was developed for the determination of total cholesterol in food samples. (C) 2013 Elsevier B.V. All rights reserved.