Photodynamic Therapy against Glioblastoma: Investigating phenoselenazine based photosensitizer as a promising therapeutic agent

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Yeşilçimen E., Karaman O., Elmazoğlu Z., Günbaş E. G.

TBS International Biochemistry Congress 2024 - 35th National Biochemistry Congress, Antalya, Türkiye, 28 Ekim - 01 Kasım 2024, cilt.49, sa.1, ss.83-84, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Cilt numarası: 49
  • Doi Numarası: 10.13140/rg.2.2.14693.72167
  • Basıldığı Şehir: Antalya
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.83-84
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Objectives: Glioblastoma Multiforme (GBM) is the

most aggressive malignant brain tumor. Photodynamic

therapy (PDT) is a promising treatment strategy

against GBM. It exerts cytotoxic effect by producing

ROS, mainly singlet oxygen, leading to cell death. The

aim of the present study was to investigate the efficacy

of a novel phenoselenazine based photosensitizer

1-(7-(azetidin-1-yl)-3H-phenoselenazin-3-ylidene)

azetidin-1-ium (NSeAze) with NIR light irradiation

on glioblastoma cells.

Methods: U-87MG and U-118MG glioblastoma cells

were treated with NSeAze for 24h to assess its dark

toxicity. For PDT application, cells were treated for

2h and exposed to 660-670 nm LED light for 1h, then

incubated overnight up to 24h in the dark. The cell viability

was detected with MTT assay following NSe-

Aze administration with/without the presence of scavengers.

The subcellular localization in mitochondria/

lysosomes was visualized by confocal microscopy.

Results: Results showed a significant reduction in cell

viability with IC50 of 376.3±17.85 nM and 531.7±25.53

nM in U-87MG and U-118MG cells, respectively. The

phototoxicity index (PI) was determined as 472.49

and 72.89, indicating the remarkable effect of NSeAze

under light irradiation. The scavenger assay confirmed

that NSeAze induces oxidative stress by producing

mostly singlet oxygen. The subcellular localization

showed that the agent predominantly accumulates in

lysosomes, with a strong Pearson correlation value of

0.78, compared to mitochondria (0.69).

Conclusions: The present study showed that the novel

photosensitizer NSeAze has significant antitumor activity

as a PDT agent against glioblastoma cells, indicating

that it may be a promising therapeutic agent in

the treatment. This study is supported by ERC 852614

project grant.

Keywords: Photodynamic Therapy, Oxidative Stress,

Glioblastoma Multiforme, Cancer, Photosensitizer