Targeted disruption of homoserine dehydrogenase gene and its effect on cephamycin C production in Streptomyces clavuligerus


Yilmaz E. I., Caydasi A. K., ÖZCENGİZ G.

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, cilt.35, sa.1, ss.1-7, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 35 Sayı: 1
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1007/s10295-007-0259-8
  • Dergi Adı: JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1-7
  • Anahtar Kelimeler: Streptomyces clavuligerus, homoserine dehydrogenase, cephamycin C, aspartate pathway, gene disruption, THRC-THRB CLUSTER, EVOLUTIONARY RELATIONSHIPS, NUCLEOTIDE-SEQUENCE, ESCHERICHIA-COLI, THREONINE GENES, AMINO-ACIDS, CLONING, BIOSYNTHESIS, ASPARTOKINASE, EXPRESSION
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

The aspartate pathway of Streptomyces clavuligerus is an important primary metabolic pathway which provides substrates for beta-lactam synthesis. In this study, the hom gene which encodes homoserine dehydrogenase was cloned from the cephamycin C producer S. clavuligerus NRRL 3585 and characterized. The fully sequenced open reading frame encodes 433 amino acids with a deduced M (r) of 44.9 kDa. The gene was heterologously expressed in the auxotroph mutant Escherichia coli CGSC 5075 and the recombinant protein was purified. The cloned gene was used to construct a plasmid containing a hom disruption cassette which was then transformed into S. clavuligerus. A hom mutant of S. clavuligerus was obtained by insertional inactivation via double crossover, and the effect of hom gene disruption on cephamycin C yield was investigated by comparing antibiotic levels in culture broths of this mutant and in the parental strain. Disruption of hom gene resulted in up to 4.3-fold and twofold increases in intracellular free L-lysine concentration and specific cephamycin C production, respectively, during stationary phase in chemically defined medium.