A dye-ligand immobilized poly(2-hydroxyethylmethacrylate) membrane used for adsorption and isolation of immunoglobulin G


Bayramoglu G., Oktem H. A. , Arica M. Y.

BIOCHEMICAL ENGINEERING JOURNAL, cilt.34, ss.147-155, 2007 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 34 Konu: 2
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1016/j.bej.2006.11.025
  • Dergi Adı: BIOCHEMICAL ENGINEERING JOURNAL
  • Sayfa Sayıları: ss.147-155

Özet

Poly(2-hydroxyethylmethaerylate) (pHEMA) membrane was prepared by photo-polymerization and a dye ligand (i.e., Reactive Green 5) was immobilised on the membrane surface (pHEMA-RG-5). Surface wettability and hydrophilicity of the dye-ligand immobilised membranes were investigated by static contact angle measurements. The chromatographic properties of the dye-ligand immobilized membrane for adsorption and purification of IgG are discussed using bare membrane as a control system. The non-specific adsorption of IgG was low (0.45 mg protein ml(-1) membrane). The attachment of dye ligand onto the membrane significantly increased the IgG adsorption (33.75 mg protein ml(-1) membrane). The maximum IgG adsorption was observed at pH 6.0. The reversible IgG adsorption on the membrane obeyed both the Freundlich and the Langmuir-Freundlich isotherm models. To test the efficiency of IgG isolation from human serum with the pHEMA-RG-5 membrane, the purity of the eluted IgG, as determined by HPLC, was 81% with a recovery of 67%. (c) 2006 Elsevier B.V. All rights reserved.