Optimal conditions for enhanced beta-mannanase production by recombinant Aspergillus sojae


Ozturk B., ÇEKMECELİOĞLU D. , Ogel Z. B.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, vol.64, pp.135-139, 2010 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 64
  • Publication Date: 2010
  • Doi Number: 10.1016/j.molcatb.2010.02.009
  • Title of Journal : JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
  • Page Numbers: pp.135-139
  • Keywords: Recombinant Aspergillus sojae, Endo-beta-1,4-mannanase, Response surface methodology, Box-Behnken, ALPHA-AMYLASE, OPTIMIZATION, PURIFICATION, ENZYMES, NIGER, ENDO-BETA-1,4-MANNANASE, HEMICELLULOSE, FERMENTATION, HYDROLYSIS, EXPRESSION

Abstract

Optimization of the growth conditions for maximum P-mannanase production in shake flasks by using recombinant Aspergillus sojae ATCC11906 (AsT1) was carried out by Box-Behnken design of response surface methodology. The highest beta-mannanase activity on the fourth day of cultivation at 30 degrees C was obtained as 363 U/ml in the optimized medium consisting of 7% sugar beet molasses, 0.43% NH(4)NO(3), 0.1% K(2)HPO(4) and 0.05% MgSO(4) (by weight per volume) at 207 rpm. On the sixth day of cultivation under the optimized conditions, the highest beta-mannanase activity was achieved as 482 U/ml which is 1.4-fold of 352 U/ml activity found on glucose medium previously. (C) 2010 Elsevier BM. All rights reserved.