Immobilization of invertase in conducting polymer matrices

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Selampinar F., Akbulut U., Ozden M., Toppare L.

BIOMATERIALS, vol.18, no.17, pp.1163-1168, 1997 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 18 Issue: 17
  • Publication Date: 1997
  • Doi Number: 10.1016/s0142-9612(97)00056-2
  • Journal Name: BIOMATERIALS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1163-1168
  • Keywords: immobilization, conducting polymer matrices, invertase, polymerization, GLUCOSE-OXIDASE, POLYPYRROLE, POLY(STYRENESULFONATE), ELECTRODES, BIOSENSORS, COMPOSITE, FILMS
  • Middle East Technical University Affiliated: Yes


This paper reports a novel approach in the electrode immobilization of an enzyme, invertase, by electrochemical polymerization of pyrrole in the presence of enzyme. The polypyrrole/invertase and polyamide/polypyrrole/invertase electrodes were constructed by the entrapment of enzyme in conducting matrices during electrochemical polymerization of pyrrole. This study involves the preparation and characterization of polypyrrole/invertase and polyamide/polypyrrole/invertase electrodes under conditions compatible with the enzyme. It demonstrates the effects of pH and temperature on the properties of enzyme electrode. Enzyme leakage tests were carried out during reuse number studies. The preparation of enzyme electrodes was done in two different electrolyte/solvent systems. The enzyme serves as a sucrose electrode and retains its activity for several months. (C) 1997 Elsevier Science Limited. All rights reserved.