Gene expression analysis of drug-resistant MCF-7 cells: implications for relation to extracellular matrix proteins


Iseri O. D. , Kars M. D. , Arpaci F., GÜNDÜZ U.

CANCER CHEMOTHERAPY AND PHARMACOLOGY, vol.65, no.3, pp.447-455, 2010 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 65 Issue: 3
  • Publication Date: 2010
  • Doi Number: 10.1007/s00280-009-1048-z
  • Title of Journal : CANCER CHEMOTHERAPY AND PHARMACOLOGY
  • Page Numbers: pp.447-455
  • Keywords: Multidrug resistance, cDNA microarray, ECM, Integrin, MMP, ADAM, HUMAN COLON-CARCINOMA, BREAST-CANCER CELLS, MULTIDRUG-RESISTANCE, P-GLYCOPROTEIN, ENDOTHELIAL-CELLS, INDUCED APOPTOSIS, TISSUE INHIBITOR, GROWTH-FACTOR, TUMOR-CELLS, FIBRONECTIN

Abstract

Since multidrug resistance is a multifactorial phenomenon, a large-scale expression analysis of drug-resistant cells by using high-density oligonucleotide microarrays may provide information about new candidate genes contributing to resistance. Extracellular matrix (ECM) is responsible for many aspects of proliferation and invasive/metastatic behavior of tumor cells. This study demonstrates alterations in gene expression levels of several ECM components, matrix metalloproteinases (MMPs), adamalysins (ADAMs and ADAMTSs) and tissue inhibitors of metalloproteinases (TIMPs) in paclitaxel, docetaxel, vincristine and doxorubicin-resistant MCF-7 cells.