Evaluation of heterotrophic and mixotrophic cultivation of novel Micractinium sp ME05 on vinasse and its scale up for biodiesel production

Engin I. K., ÇEKMECELİOĞLU D., YÜCEL A. M., ÖKTEM H. A.

BIORESOURCE TECHNOLOGY, cilt.251, ss.128-134, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 251
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1016/j.biortech.2017.12.023
  • Dergi Adı: BIORESOURCE TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.128-134
  • Anahtar Kelimeler: Vinasse, Heterotrophic, Mixotrophic, Micractinium sp, FAME, WASTE-WATER, SUGARCANE VINASSE, NUTRIENT REMOVAL, BIOFUEL PRODUCTION, SPIRULINA-MAXIMA, SCENEDESMUS SP, BEET VINASSE, MICROALGAE, GROWTH, BIOMASS
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Direct disposal of vinasse, a by-product of molasses fermentation plants, threatens environmental health. This study investigated the usage of vinasse as a nutrient source for the heterotrophic and mixotrophic cultivation of novel Micractinium sp. ME05. The 500-mL flask experiments resulted in higher biomass productivities under mixotrophic conditions (0.16 +/- 0.01 g L-1 day(1)) than the heterotrophic conditions (0.13 +/- 0.01 g L-1 day(1)). A 1.7-fold increase in biomass productivity was achieved by scaling up from 500-mL flasks (0.16 +/- 0.01 g L-1 day(1)) to 2-L flasks (0.27 +/- 0.019 g L-1 day(1)). The 5-L bioreactor experiments resulted in a biomass productivity of 0.32 +/- 0.2 g L-1 day(1) and lipid productivity of 3.4 +/- 0.20 g L-1 day(-1). This study demonstrated that Micractinium sp. ME05 can be cultivated with vinasse to produce large amounts of biomass. The FAME profile of mixotrophic Micractinium sp. ME05 cells was promising for further biodiesel production. This study highlights the feasibility of industrial by-product-vinasse as the nutrient source for biomass and lipid productions using the novel Micractinium sp. ME05 cells.