A multiplex primer design algorithm for target amplification of continuous genomic regions

OZTURK A. R., CAN T.

BMC BIOINFORMATICS, vol.18, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 18
  • Publication Date: 2017
  • Doi Number: 10.1186/s12859-017-1716-7
  • Journal Name: BMC BIOINFORMATICS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: Next Generation sequencing, Target amplification, Multiplex PCR, Primer design, MUTATION DETECTION, ENRICHMENT
  • Middle East Technical University Affiliated: Yes

Abstract

Background: Targeted Next Generation Sequencing (NGS) assays are cost-efficient and reliable alternatives to Sanger sequencing. For sequencing of very large set of genes, the target enrichment approach is suitable. However, for smaller genomic regions, the target amplification method is more efficient than both the target enrichment method and Sanger sequencing. The major difficulty of the target amplification method is the preparation of amplicons, regarding required time, equipment, and labor. Multiplex PCR (MPCR) is a good solution for the mentioned problems.