Immunogenicity and protective efficacy of the recombinant Pasteurella lipoprotein E and outer membrane protein H from Pasteurella multocida A:3 in mice


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Okay S., Ozcengiz E., GÜRSEL İ., ÖZCENGİZ G.

RESEARCH IN VETERINARY SCIENCE, vol.93, no.3, pp.1261-1265, 2012 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 93 Issue: 3
  • Publication Date: 2012
  • Doi Number: 10.1016/j.rvsc.2012.05.011
  • Title of Journal : RESEARCH IN VETERINARY SCIENCE
  • Page Numbers: pp.1261-1265
  • Keywords: Outer membrane protein H, Pasteurella lipoprotein E, Pasteurella multocida, Recombinant vaccine, GENE OMPH, VACCINE, CHALLENGE, RESPONSES, IMMUNITY, CLONING

Abstract

Pasteurella multocida serotype A:3 is a Gram-negative bacterial pathogen, one of the causative agents of shipping fever of cattle. In this study, outer membrane protein H (ompH) and Pasteurella lipoprotein E (plpE) genes were cloned and plpEC-ompH fusion was constructed and expressed in Escherichia coli. Recombinant PlpE, OmpH and PlpEC-OmpH fusion proteins were purified and formulated with oil-based and oil-based CpG ODN adjuvants. Antibody responses in mice vaccinated with recombinant PlpE and PlpEC-OmpH proteins formulated with both adjuvants were significantly (p < 0.05) increased. However, a significant (p < 0.05) increment in serum IFN-gamma level was only observed upon immunization with oil-based CpG formulations. Protectivity of the vaccines were evaluated via intraperitoneal challenge of mice with 10 LD50 of P. multocida A:3. The recombinant proteins PlpE and PlpEC-OmpH fusion conferred 100% protection when formulated with oil-based CpG ODN while the protectivity was found to be 80% and 60%, respectively when only oil-based adjuvant was used in respective formulations. These findings indicated that the recombinant PIPE or PlpEC-OmpH fusion proteins formulated with oil-based CpG ODN adjuvant are possible acellular vaccine candidates against shipping fever. (C) 2012 Elsevier Ltd. All rights reserved.