Kinetic analysis of photosynthetic growth, hydrogen production and dual substrate utilization by Rhodobacter capsulatus

Sevinc P., GÜNDÜZ U., EROĞLU İ., Yucel M.

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, vol.37, no.21, pp.16430-16436, 2012 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 21
  • Publication Date: 2012
  • Doi Number: 10.1016/j.ijhydene.2012.02.176
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.16430-16436
  • Keywords: Biological hydrogen production, Rhodobacter capsulatus, Dual substrate consumption, Kinetic analysis, Photofermentation, BIOHYDROGEN PRODUCTION, FERMENTATION, METABOLISM, PYRUVATE


Rhodobacter capsulatus is purple non-sulfur (PNS) bacterium which can produce hydrogen and CO2 by utilizing volatile organic acids in presence of light under anaerobic conditions. Photofermentation by PNS bacteria is strongly affected by temperature and light intensity. In the present study we present the kinetic analysis of growth, hydrogen production, and dual consumption of acetic acid and lactic acid at different temperatures (20, 30 and 38 degrees C) and light intensities (1500, 2000, 3000, 4000 and 5000 lux). The cell growth data fitted well to the logistic model and the cumulative hydrogen production data fitted well to the Modified Gompertz Model. The model parameters were affected by temperature and light intensity. Lactic acid was found to be consumed by first order kinetics. Rate of consumption of acetic acid was zero order until most of the lactic acid was consumed, and then it shifted to first order. The results revealed that the optimum light intensities for maximum hydrogen production were 5000 lux for 20 degrees C and 3000 lux for 30 degrees C and 38 degrees C. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.