Evaluation of methylation profiles of an epidermal growth factor receptor gene in a head and neck squamous cell carcinoma patient group

Mutlu M., MUTLU P., Azarkan S., Baylr Ö., Öcal B., Saylam G., ...More

Balkan Journal of Medical Genetics, vol.23, no.2, pp.65-72, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 2
  • Publication Date: 2021
  • Doi Number: 10.2478/bjmg-2020-0025
  • Journal Name: Balkan Journal of Medical Genetics
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, Directory of Open Access Journals
  • Page Numbers: pp.65-72
  • Keywords: Epidermal growth factor receptor (EFGR) gene, epigenetics, head and neck squamous cell carcinoma (HNSCC), methylation, methylation-specific-polymerase chain reaction (MS-PCR)
  • Middle East Technical University Affiliated: Yes


© 2020 Mutlu M, Mutlu P, Azarkan S, Baylr Ö, Öcal B, Saylam G, Korkmaz MH, published by Sciendo 2020.Upregulation of the epidermal growth factor receptor (EGFR) gene has shown an important impact on the development of head and neck cancers due to its important regulation role on multiple cell signaling pathways. The aim of this study was to investigate the methylation pattern of the promoter region of the EGFR gene between head and neck squamous cell carcinoma (HNSCC) patients and a control group. Forty-seven unrelated HNSCC patients, clinically diagnosed at the Department of Otorhinolaryngology, Dlşkapl Ylldlrlm Beyazlt Training and Research Hospital, Ankara, Turkey, and 48 unrelated healthy volunteers from different geographic regions of Turkey, were included in this study. Methylation status of the promoter region of the EGFR gene was detected by methylation-specific-polymerase chain reaction (MS-PCR). The correlation between EGFR gene promoter methylation profiles and clinical characteristics were examined using the χ2 test. Methylation was observed in 79.0% of HNSCC patients, whereas this ratio was 90.0% in healthy individuals. The results show that promoter region methylation of the EGFR gene was not associated with HNSCC development in the studied Turkish patient group. In addition, the methylation status of the EGFR gene promoter was not found to be related to age, gender or tumor stage.