Various applications of immobilized glucose oxidase and polyphenol oxidase in a conducting polymer matrix

Cil M., Boyukbayram A. E., Kiralp S., Toppare L., Yagci Y.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.41, sa.1, ss.49-55, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 1
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1016/j.ijbiomac.2006.12.005
  • Dergi Adı: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.49-55
  • Anahtar Kelimeler: electrochemical polymerization, glucose oxidase, polyphenol oxidase, TYROSINASE, COPOLYMERS, ELECTRODES, ENZYMES
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

In this study, glucose oxidase and polyphenol oxidase were immobilized in conducting polymer matrices; polypyrrole and poly(N-(4-(3-thienyl methylene)-oxycarbonyl phenyl) maleimide-co-pyrrole) via electrochemical method. Fourier transform infrared and scanning electron microscope were employed to characterize the copolymer of (N-(4-(3-thienyl methylene)-oxycarbonyl phenyl) maleimide) with pyrrole. Kinetic parameters, maximum reaction rate and Michealis-Menten constant, were determined. Effects of temperature and pH were examined for immobilized enzymes. Also, storage and operational stabilities of enzyme electrodes were investigated. Glucose and polyphenol oxidase enzyme electrodes were used for determination of the glucose amount in orange juices and human serum and phenolic amount in red wines, respectively. (c) 2007 Elsevier B.V. All rights reserved.