A New Amperometric Alcohol Oxidase Biosensor Based on Conducting Polymer of (4,7-Dithien-2-yl-2, 1,3-benzothiadiazole)
JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY, vol.49, no.3, pp.185-190, 2012 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 49 Issue: 3
- Publication Date: 2012
- Doi Number: 10.1080/10601325.2012.649210
- Journal Name: JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.185-190
- Middle East Technical University Affiliated: Yes
Abstract
Amperometric biosensors based on alcohol oxidase (AOX) immobilized on the conducting polymers of 3,4-ethylenedioxythiophene (PEDOT) and 4,7-dithien-2-yl-2,1,3-benzothiadiazole (TBTD) were constructed. Proposed biosensors were characterized using ethanol as the substrate and the responses were measured. Kinetic parameters, I-max, K-m and sensitivity were determined for the biosensors with or without TBTD. Optimization studies for TBTD thickness, PEDOT amount and pH were carried out. The biosensing capacity was discussed for TBTD matrix.