A New Amperometric Alcohol Oxidase Biosensor Based on Conducting Polymer of (4,7-Dithien-2-yl-2, 1,3-benzothiadiazole)
JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY, cilt.49, sa.3, ss.185-190, 2012 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 49 Sayı: 3
- Basım Tarihi: 2012
- Doi Numarası: 10.1080/10601325.2012.649210
- Dergi Adı: JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.185-190
- Orta Doğu Teknik Üniversitesi Adresli: Evet
Özet
Amperometric biosensors based on alcohol oxidase (AOX) immobilized on the conducting polymers of 3,4-ethylenedioxythiophene (PEDOT) and 4,7-dithien-2-yl-2,1,3-benzothiadiazole (TBTD) were constructed. Proposed biosensors were characterized using ethanol as the substrate and the responses were measured. Kinetic parameters, I-max, K-m and sensitivity were determined for the biosensors with or without TBTD. Optimization studies for TBTD thickness, PEDOT amount and pH were carried out. The biosensing capacity was discussed for TBTD matrix.