Agomelatine strongly interacts with zwitterionic DPPC and charged DPPG membranes

Ergun S., Demir P., Uzbay T., SEVERCAN F.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, cilt.1838, sa.11, ss.2798-2806, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1838 Sayı: 11
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.bbamem.2014.07.025
  • Dergi Adı: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2798-2806
  • Anahtar Kelimeler: Agomelatine, Membrane lipid, FTIR spectroscopy, DSC, Lipid structure, DIFFERENTIAL SCANNING CALORIMETRY, TRANSFORM INFRARED-SPECTROSCOPY, MODEL MEMBRANES, PHOSPHOLIPID MONOLAYERS, PHOSPHATIDYLCHOLINE MEMBRANES, PHASE-TRANSITION, OXIDATIVE STRESS, LIPID-BILAYER, DYNAMICS, CHOLESTEROL
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Depression is one of the most common psychiatric diseases in the population. Agomelatine is a novel antidepressant drug with melatonin receptor agonistic and serotonin 5-HT2C antagonistic properties. Furthermore, being a melatonergic drug, agomelatine has the potential of being used in therapeutic applications like melatonin as an antioxidant, anti-inflammatory and antiapoptotic drug. The action mechanism of agomelatine on the membrane structure has not been clarified yet. In the present study, we aimed to investigate the interaction of agomelatine with model membranes of dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylgylcerol (DPPG) by Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC). We found that agomelatine interacts with the head group in such a manner that it destabilizes the membrane architecture to a large extent. Thus, agomelatine causes alterations in the order, packing and dynamics of the DPPC and DPPG model membranes. Our results suggest that agomelatine strongly interacts with zwitterionic and charged membrane phospholipids. Because lipid structure and dynamics may have influence on the structure of membrane bound proteins and affect the signal transduction systems of membranes, these effects of agomelatine may be important in its action mechanism. (C) 2014 Elsevier B.V. All rights reserved.