Biosensors Based on Nano-Gold/Zeolite-Modified Ion Selective Field-Effect Transistors for Creatinine Detection

Kasap B. O., Marchenko S. V., Soldatkin O. O., Dzyadevych S. V., AKATA KURÇ B.

NANOSCALE RESEARCH LETTERS, vol.12, 2017 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 12
  • Publication Date: 2017
  • Doi Number: 10.1186/s11671-017-1943-x
  • Journal Name: NANOSCALE RESEARCH LETTERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: Zeolite, Enzyme, Biosensor, Creatinine deiminase, ISFET, BEA, Silicalite, Gold nanoparticle, NANOSIZED MICRO/MESOPOROUS COMPOSITES, SENSITIVE BIOSENSOR, PROTEIN ADSORPTION, ENZYMATIC-ACTIVITY, EFFECT DEVICES, ZEOLITE, GOLD, IMMOBILIZATION, FABRICATION, PH
  • Middle East Technical University Affiliated: Yes

Abstract

The combination of advantages of using zeolites and gold nanoparticles were aimed to be used for the first time to improve the characteristic properties of ion selective field-effect transistor (ISFET)-based creatinine biosensors. The biosensors with covalently cross-linked creatinine deiminase using glutaraldehyde (GA) were used as a control group, and the effect of different types of zeolites on biosensor responses was investigated in detail by using silicalite, zeolite beta (BEA), nano-sized zeolite beta (Nano BEA) and zeolite BEA including gold nanoparticle (BEA-Gold). The presence of gold nanoparticles was investigated by ICP, STEM-EDX and XPS analysis. The chosen zeolite types allowed investigating the effect of aluminium in the zeolite framework, particle size and the presence of gold nanoparticles in the zeolitic framework.