Development of Random Amplified Polymorphic DNA (RAPD) markers in 150 F-6-inbred durum wheat (Triticum durum) lines derived from Kunduru-1149 x Cham-1 cross

Gocmen B., Keskin S., Kaya Z., Taskin V.

ISRAEL JOURNAL OF PLANT SCIENCES, vol.51, no.4, pp.245-249, 2003 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 51 Issue: 4
  • Publication Date: 2003
  • Doi Number: 10.1560/3479-kda6-ffgt-myjn
  • Journal Name: ISRAEL JOURNAL OF PLANT SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.245-249
  • Middle East Technical University Affiliated: Yes

Abstract

Random Amplified Polymorphic DNA (RAPD) markers were developed in a specific durum wheat population (150 lines) derived from Kunduru-1149 x Chain-1 cross. Of 284 RAPD primers screened against parent DNAs, 83 revealed at least one polymorphic locus. The most polymorphic and clear bands producing 13 primers were selected and screened with the DNAs of 150 lines. These 13 primers revealed a total number of 33 segregating loci. The preliminary results of this study could be used as a base for mapping economically important character loci of durum wheat by the development of other RAPDs.