Conducting polymers with benzothiadiazole and benzoselenadiazole units for biosensor applications

Emre F. B., Ekiz F., Balan A., Emre S., TİMUR S., TOPPARE L. K.

SENSORS AND ACTUATORS B-CHEMICAL, vol.158, no.1, pp.117-123, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 158 Issue: 1
  • Publication Date: 2011
  • Doi Number: 10.1016/j.snb.2011.05.052
  • Journal Name: SENSORS AND ACTUATORS B-CHEMICAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.117-123
  • Keywords: Enzyme immobilization, Conducting polymer, Biosensor, Surface modification, AMPEROMETRIC GLUCOSE BIOSENSOR, PI-STACKING INTERACTIONS, 7-METHYLGUANINE BASE, NEUTRAL STATE, IMMOBILIZATION, OXIDASE, FILM
  • Middle East Technical University Affiliated: Yes

Abstract

Poly(4,7-di(2,3)-dihydrothienol[3,4-b][1,4]dioxin-5-yl-benzo[1,2,5]thiadiazole) (PBDT) and poly(4,7-di(2,3)-dihydrothienol[3,4-b][1,4]dioxin-5-yl-2,1,3-benzoselenadiazole) (PESeE) were electrochemically deposited on graphite electrodes and used as immobilization matrices for biosensing studies. After electrochemical deposition of the polymeric matrices, glucose oxidase (GOx) was immobilized on the modified electrodes as the model enzyme. In the biosensing studies, the decrease in oxygen level as a result of enzymatic reaction was monitored at -0.7 V vs Ag/AgCl (3.0 M KCl) and correlated with substrate concentration. The biosensor was characterized in terms of several parameters such as operational and storage stabilities, kinetic parameters (K(m) and I(max)) and surface morphologies. The biosensor was tested on real human blood serum samples. (C) 2011 Elsevier B.V. All rights reserved.