Poly-beta-hydroxybutyrate accumulation and releasing by hydrogen producing bacteria, Rhodobacter sphaeroides OU001. A transmission electron microscopic study

Cetin D., Guenduez U., Eroglu i., Yuecel M., Tuerker L.

AFRICAN JOURNAL OF BIOTECHNOLOGY, cilt.5, sa.22, ss.2069-2072, 2006 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 5 Sayı: 22
  • Basım Tarihi: 2006
  • Dergi Adı: AFRICAN JOURNAL OF BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, Pollution Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.2069-2072
  • Anahtar Kelimeler: poly-beta-hydroxybutyrate, PHB, Rhodobacter sphaeroides, transmission electron microscopy, ESCHERICHIA-COLI, SUGAR REFINERY, WASTE-WATER, OU 001, PHOTOPRODUCTION, GRANULES, POLYHYDROXYALKANOATES, BIOSYNTHESIS, CARBON, PH
  • Orta Doğu Teknik Üniversitesi Adresli: Hayır

Özet

Photosynthetic bacterium Rhodobacter sphaeroides O.U.001 that is used for photobiohydrogen production can also accumulate poly-beta-hydroxybutyrate (PHB) as a by-product when cultivated anaerobically with minimal medium containing L-malic acid, sodium glutamate and some vitamins under illumination. Transmission electron microscopy studies revealed that PHB granules are made of two distinct components: a homogenous and electron lucent core is covered with a more electron dense coat. PHB granules were observed in the cytoplasm, outside of the cells, in the center of cotton like aggregates in the cells, or while they were being released from the cell. In this study, two kinds of releasing were revealed; with lysis and without lysis of cell wall. Release of intact polymer outside the cells could be economically feasible way to obtain PHB for industrial applications.