Magnitude and efficiency of genetic diversity captured from seed stands of Pinus nigra (Arnold) subsp pallasiana in established seed orchards and plantations

Cengel B., Tayanc Y., Kandemir G., Velioglu E., Alan M., KAYA Z.

NEW FORESTS, cilt.43, sa.3, ss.303-317, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43 Sayı: 3
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1007/s11056-011-9282-8
  • Dergi Adı: NEW FORESTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.303-317
  • Anahtar Kelimeler: Anatolian black pine, Pinus nigra subspecies pallasiana, RAPD, Seed stand, Seed orchard, Plantation, Genetic diversity, Forestry practices, PICEA-ABIES KARST, DNA RAPD MARKERS, PHYLOGENETIC TREES, FOREST MANAGEMENT, CHLOROPLAST DNA, NATURAL STANDS, POPULATIONS, ALLOZYME, MICROSATELLITE, CONSERVATION
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

To test how efficiently plantations and seed orchards captured genetic diversity from natural Anatolian black pine (Pinus nigra Arnold subspecies pallasiana Holmboe) seed stands, seed sources were chosen from 3 different categories (seed stands (SS), seed orchards (SO) and plantations (P)) comprising 4 different breeding zones of the species in Turkey. Twenty-five trees (mother trees) were selected from each SS, SO and P seed sources and were screened with 11 Random Amplified Polymorphic DNA (RAPD) markers. Estimated genetic diversity parameters were found to be generally high in all Anatolian black pine seed sources and the majority of genetic diversity is contained within seed sources (94%). No significant difference in genetic diversity parameters (numbers of effective alleles, % of polymorphic loci and heterozygosity) among seed source categories was found, except for a slight increase in observed heterozygosities in seed orchards. For all seed source categories, observed heterozygosity values were higher (H-o = 0.49 for SS, 0.55 for SO and 0.49 for P) than expected ones (H-e = 0.40 for SS, 0.39 for SO and 0.38 for P) indicating the excess of heterozygotes. In general, genetic diversity in seed stands has been transferred successfully into seed orchards and plantations. However, the use of seeds from seed orchards can increase the amount of genetic diversity in plantations further. The study also demonstrated that number of plus-tree clones (25-38) used in the establishment of seed orchards was adequate to capture the high level of diversity from natural stands.